E2264

Endoglycosidase F3 from Elizabethkingia miricola

recombinant, expressed in E. coli, 30 U/mg

• Synonym(e): Elizabethkingia miricola, Endo-β-N-acetylglucosaminidase F3, Endoglycosidase F3 from Elizabethkingia (Chryseobacterium/Flavobacterium) meningosepticum
• EC-Nummer: 3.2.1.96 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00146843
• UNSPSC-Code: 12352204
• NACRES: NA.32

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualitätsniveau: 200
• Konjugat: (N-linked)
• Form: solution
• Spezifische Aktivität: 30 U/mg
• Mol-Gew.: 32 kDa
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C

Beschreibung

Anwendung

Endoglycosidase F3 from Elizabethkingia miricola has been used to analyze core fucosylation and tryptic digests of serum proteins.

Biochem./physiol. Wirkung

Endoglycosidase F3 belongs to the glycoside hydrolase family 18 (GH18). It has hydrolytic activity. Endoglycosidase F3 glycosylates α-1,6-fucosylated GlcNAc derivative to give natural, core fucosylated complex-type N-glycopeptides.

Cleaves asparagine-linked biantennary and triantennary complex, oligosaccharides depending on the state of core fucosylation and peptide linkage.

Verpackung

Supplied with 5× Reaction Buffer, 250 mM sodium acetate, pH 4.5

Einheitendefinition

One unit will release N-linked oligosaccharides from 1 μmole of denatured porcine fibrinogen in 1 minute at 37 °C, pH 4.5.

Physikalische Form

Aseptically filled solution in 20 mM Tris-HCl, pH 7.5

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)