MFE-296

98031101, human endometrium, Epithelial

• Synonym(e): MFE296 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• product name: MFE-296, 98031101
• Biologische Quelle: human endometrium
• Wachstumsmodus: Adherent
• Karyotyp: Near Tetraploid
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Androgen receptor
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human caucasian endometrial adenocarcinoma

Beschreibung der Zelllinie

MFE-296 was obtained from a moderately differentiated human endometrial adenocarcinoma. The cells were shown to express functionally intact androgen receptors. Proliferation was inhibted by progestin R5020 and dihydrotestosterone (DHT), the latter one being antagonised by the antiandrogen Casodex. MFE-296 express vimentin and the cytokeratins 7, 8, 18 and 19. Karyotype analysis revealed near tetraploidy for most cells. Further more, the cells have been shown to be tumorigenic in nude mice.

Anwendung

Tumour biology studies, receptor analysis

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 8,13
D16S539: 11,14
D5S818: 11,13
D7S820: 8,10
THO1: 6,9.3
TPOX: 8
vWA: 14,18

Nährmedium

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding 1-2 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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