CAR, ML human cell line

94082207, IHW Number: 9108

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human blood
• Ethnizität: Not specified
• Geschlecht: not specified
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Lymphoblastoid
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice

Beschreibung

Allgemeine Beschreibung

Consanguineous: No

Homozygous: No

Beschreibung der Zelllinie

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA-Profil

Cw: 401
HLA DRB1: 0101 1401
HLA DRB3: 202
HLA DQA1: 101
HLA DQB1: 501
HLA DPB1: 0401 0402

Serologisches Profil

HLA A: 2
HLA C: 4 3
HLA B: 35 55
Bw4/bw6: 6

Nährmedium

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subkultur-Routine

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Sonstige Hinweise

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Haftungsausschluss

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.