Detroit 510

90102531, human skin, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: Detroit 510, 90102531
• Biologische Quelle: human skin
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 46
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian skin Galactosemia

Beschreibung der Zelllinie

Derived from skin tissue of a 9 month old Caucasian female with Galactosemia. Galactose-1-phosphate transferase negative.

Anwendung

Virus studies

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 10,12
D7S820: 8,12
THO1: 7,9.3
TPOX: 8
vWA: 15,17

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10%Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Finite lifespan, up to 30 population doublings are expected.

Sonstige Hinweise

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