A375 Cell Line human

88113005, human skin, Epithelial

• Synonym(e): A-375 Cells, A375-MEL Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: A375 Cell Line human, 88113005
• Biologische Quelle: human skin
• Beschreibung: Human malignant melanoma
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. = 62 (hypotriploid) 9 marker chromosomes. Normal N2, N6 and N22 present.
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human malignant melanoma

Beschreibung der Zelllinie

Derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice.

Anwendung

A375 cell line has been used to obtain paracrine factors for prolonged culture of mesenchymal stromal cells (MSCs). It has also been used to study oncolytic activity of the peptide LTX-315.

Tumourigenicity studies

Nährmedium

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Sonstige Hinweise

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