RPMI 2650

88031602, human nose, Epithelial

• Synonym(e): RPMI Cell Culture Medium
• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: RPMI 2650, 88031602
• Biologische Quelle: human nose
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 46
• Morphologie: Epithelial
• Produkte: Mucoid material
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human nasal septum quasidiploid tumour

Beschreibung der Zelllinie

From the pleural effusion of an extensive malignant tumour of the nasal septum, diagnosed as anaplastic squamous cell carcinoma. Nearly normal karyotype, stability of chromosome number and probable origin from malignant cells make the cell line unique amongst permanent human cell lines. Cells are very small and grow in clusters fusing to form thick layers.

Anwendung

Tumourigenicity studies

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 9,11
D13S317: 11,12
D16S539: 11,12
D5S818: 12,13
D7S820: 8,11
THO1: 6,8
TPOX: 8
vWA: 16,18

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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