SP2/O-Ag14 Cell Line murine

Non-producing hybridoma, 85072401

• Synonym(e): Mouse Cell Line
• UNSPSC-Code: 12352207

Eigenschaften

• Biologische Quelle: mouse spleen
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Not specified
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse x Mouse myeloma, non-producing

Beschreibung der Zelllinie

Sp2/0-Ag14 is a non-Ig-secreting or synthesising line derived from a cell line created by fusing a BALB/c mouse spleen cell and the mouse myeloma P3X63Ag8. Resistant to 8-azaguanine at 20ug/ml and does not survive in HAT containing media. HAT sensitive mouse myeloma hybrid suitable for use as a hybridoma fusion partner - in this sense, functionally it is a myeloma cell line and often referred to as such in the literature.

Anwendung

Fusion partner

Nährmedium

RPMI 1640 or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. HAT sensitive.

Sonstige Hinweise

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