MM466

12070507

• Synonym(e): MM 466
• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human skin (metastasis)
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Rezeptoren: Not specified
• Relevante Krankheit(en): metastasis

Beschreibung

Ursprung der Zelllinie

Human melanoma metastatic site lymph node

Beschreibung der Zelllinie

mM466 is a human melanoma derived from a metastatic site (lymph node). Cell Bank Australia reference number CBA-1353.

DNA-Profil

Not specified

Nährmedium

RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + 10% FBS.

Subkultur-Routine

Optimal split ratio 1:2-1:4 (seeding density 1.4 x 10⁴ cells/cm²). Cells incubated at 37 °C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min. Medium change twice weekly. The cell line MM466 may take up to 72 hours to recover from thaw.

Sonstige Hinweise

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Peter Parsons - Queensland Institute of Medical Research, Australia