VP303

05092806

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human breast
• Wachstumsmodus: Adherent
• Karyotyp: Mean no. 64, modal no. 67, range 57-72
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Breast Cancer

Beschreibung der Zelllinie

Derived from a breast cancer biopsy removed from a 69 year-old female. The histology of the breast cancer biopsy was defined as Ductal Grade II, 90% of tumour cells stained positive for oestrogen receptors and cells were negative for progesterone receptor staining. The VP303 cell line derived from the tumour did not stain positive for oestrogen receptors. The individual was recorded to be alive 5 years after surgery performed to remove the tumour. This cell line grows slowly and is relatively difficult to culture; please read the sub-culture routine information closely.

Nährmedium

MEBM (Clonetics, CC-3151) + MEGM SingleQuots (Clonetics, CC-4136) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS). Fresh EGF (final concentration of 10ng/ml) should be added to the medium at each subculture.

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. The VP series of breast cancer cell lines are very slow growing and relatively difficult to grow. Fresh EGF (final concentration 10ng/ml) s

Sonstige Hinweise

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