IIC9 (CH0)

30814, hamster embryo, Fibroblast

• UNSPSC-Code: 41106514
• Biologische Quelle: hamster embryo
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: PAR-1 (protease-activated receptor 1, thrombin receptor), EGF, PDGF

Eigenschaften

• Produktbezeichnung: IIC9 (CH0), 00030814
• Biologische Quelle: hamster embryo
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: PAR-1 (protease-activated receptor 1, thrombin receptor), EGF, PDGF
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Chinese hamster embryo fibroblast

Beschreibung der Zelllinie

The Chinese hamster embryo fibroblast (CHEF) line is a nontumorigenic and stable diploid cell line, with a high serum requirement for growth. It is isolated from embryo preparation and is a spontaneous tumorigenic anchorage-independent line.

Anwendung

IIC9 (CH0) is suitable for selective growth of some rodent epithelial cells in a medium containing citrulline.

Nährmedium

DMEM:F12 (1:1) (D8062) + 5% FBS / FCS (F2442) + 2mM L-Glutamine (G7513).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:10 ie. seeding at 2-4 x10,000 cells/cm² using 0.25% trypsin /EDTA; 37°C; 5% CO₂.

Sonstige Hinweise

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