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Merck

C6974

Sigma-Aldrich

Anti-CaM Kinase IIα (CaMKIIα) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.44

Biologische Quelle

rabbit

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

IgG fraction of antiserum

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Mol-Gew.

antigen 50 kDa

Speziesreaktivität

human, chicken, rat, mouse

Verpackung

antibody small pack of 25 μL

Methode(n)

microarray: suitable
western blot: 1:5,000 using synaptosomal fraction of rat brain

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... CAMK2A(815)
mouse ... Camk2a(12322)
rat ... Camk2a(25400)

Allgemeine Beschreibung

Ca2+/Calmodulin dependent protein kinase II (CaMKII) belongs to the family of Ser/Thr protein kinases including CaMKI and CaMKIV. CaM kinase II is one of the most abundant protein kinases in the mammalian brain, with the highest expression in neurons of the hippocampus and the cerebral cortex. CaMKII consists of a family of four related isoforms CaMK IIα, CaMK IIβ, CaMK IIγ and CaMK IIδ. The CaMK II α and β isoforms are predominantly expressed in the brain, localized mainly in the cytosol and postsynaptic densities (PSDs), whereas the CaMKII γ and δ isoforms are expressed in all tissues. CaMKII contains a catalytic and regulatory domain. The regulatory domain consists of an autoinhibitory and calmodulin binding site.

Spezifität

The antibody does not cross-react with CaMKIIβ, γ and δ isoforms (60 kDa). Anti-CaM Kinase IIα specifically binds to CaM Kinase IIα (50kDa) in human, mouse, rat and chicken. CaM Kinase IIα immunizing peptide specifically inhibits the staining of CaM Kinase IIα in immunoblotting.

Immunogen

Peptide corresponding to amino acids 461-478 of rat CaM Kinase Iiα, conjugated to KLH. This sequence is identical in human, mouse and chicken CaMKIIα has limited homology (50-60%) with CaM Kinase IIβ, γ and δ subunits.

Anwendung

Anti-CaM Kinase IIα (CaMKIIα) antibody has been used:
  • in immunolabelling
  • in western blotting
  • in immunoprecipitation
  • in immunoblotting

Biochem./physiol. Wirkung

CaMKII is involved in many cellular functions in response to Ca2+ signaling, including synthesis and secretion of neurotransmitters, axonal transport, long term potentiation (LTP) and spatial learning, receptor function and regulation of gene expression. CaMKII plays a critical role in LTP, a cellular model of learning and memory. In the CNS, postsynaptic Ca2+ influx triggers rapid autophosphorylation and stable activation of CaMKII in a Ca2+/calmodulin dependent manner at a threonine residue in the autoinhibitory domain. Autophosphorylation of CaMKIIα at Thr286 has been shown to be required for LTP and learning.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Empfehlung

Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Martin W Berchtold et al.
The Journal of biological chemistry, 291(52), 26636-26646 (2016-11-07)
Calmodulin (CaM) is a Ca
Tianzhi Huang et al.
Nature communications, 7, 12885-12885 (2016-10-05)
Molecularly defined subclassification is associated with phenotypic malignancy of glioblastoma (GBM). However, current understanding of the molecular basis of subclass conversion that is often involved in GBM recurrence remain rudimentary at best. Here we report that canonical Wnt signalling that
Structure of the autoinhibited kinase domain of CaMKII and SAXS analysis of the holoenzyme
Rosenberg O S, et al.
Cell, 123(5), 849-860 (2005)
CaMKII beta functions as an F-actin targeting module that localizes CaMKII alpha beta heterooligomers to dendritic spines
Shen K, et al.
Neuron, 21(3), 593-606 (1998)
Yu-Chih Lin et al.
Communicative & integrative biology, 2(1), 40-41 (2009-08-26)
CaMKII, calcium/calmodulin dependent protein kinase, is an active kinase in the cell that phosphorylates a number of substrates including several cytoskeletal and signaling proteins. In addition to kinase activity, the beta isoform of CaMKII also contains an F-actin binding region.

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