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Key Documents

C1655

Sigma-Aldrich

Choleratoxin-B-Untereinheit

FITC conjugate, lyophilized powder

Synonym(e):

CTB

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About This Item

MDL-Nummer:
MFCD02356522
UNSPSC-Code:
12352200
PubChem Substanz-ID:
329774902

Konjugat

FITC conjugate

Qualitätsniveau

200

Form

lyophilized powder

Mol-Gew.

~12 kDa

Zusammensetzung

Protein, ~20% Lowry

Kennzeichnungsgrad

~1.0 mol FITC per mol protein

Lagertemp.

2-8°C

SMILES String

CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F

InChI

1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)

InChIKey

YDXZSNHARVUYNM-UHFFFAOYSA-N

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Anwendung

Cholera Toxin B subunit-fluorescein isothiocyanate (FITC) conjugate has been used as a fluorescent tracer:
  • to label distal cerebrospinal fluid-contacting neurons (dCSF-CNs) in lateral ventricles of rat
  • to stain epididymal sperms of mice
  • to stain peritoneal macrophages of mice

Biochem./physiol. Wirkung

Die Choleratoxin-B-Untereinheit wird in der neurologischen Forschung für das Track-Tracing verwendet; dies macht sich die GM1-Gangliosidbindung und den retrograden Transport zunutze. Mit Choleratoxin behandelte Gewebekulturzellen werden nicht abgetötet, und Gewebe von Tieren werden nicht nekrotisch.

Leistungsmerkmale und Vorteile

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Physikalische Form

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium EDTA and sodium azide

Hinweis zur Analyse

Activity measured by ELISA using ganglioside GM1 coated multiwell plates, rabbit anti-cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the second antibody. 50% saturation of binding was achieved with 0.01-1 μg of the cholera toxin B subunit-FITC conjugate per mL.

Piktogramme

Exclamation mark
GHS07

Signalwort

Warning

H-Sätze

H332 - H412

Gefahreneinstufungen

Acute Tox. 4 Inhalation - Aquatic Chronic 3

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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