C0954
Chondroitinase C from Flavobacterium heparinum
lyophilized powder, ≥200 units/mg solid
Synonym(e):
Chondroitin C lyase
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Alle Fotos(2)
About This Item
Empfohlene Produkte
Konjugat
(Glucosaminoglycan)
Qualitätsniveau
Form
lyophilized powder
Spezifische Aktivität
≥200 units/mg solid
Lagertemp.
−20°C
Allgemeine Beschreibung
Enzyme Commission (EC) Number 4.2.2.x
Biochem./physiol. Wirkung
Chondroitinase C acts upon chondroitin sulfate C, releasing unsaturated 6-sulfated disaccharides. The enzyme also acts on hyaluronic acid, producing unsaturated nonsulfated disaccharides. The enzyme is strongly inhibited by NaCl, Fe2+, Co2+ and phosphate ions.
Einheitendefinition
One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 8.0 at 25 °C using chondroitin sulfate C as substrate.
Sonstige Hinweise
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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Y M Michelacci et al.
The Journal of biological chemistry, 251(4), 1154-1158 (1976-02-25)
A chondroitinase that acts upon chondroitin sulfate C and hyaluronic acid was isolated from Flavobacterium heparinum. This enzyme was seperated from constitutional chondroitinase AC and an induced chondroitinase B also present in extracts of F. heparinum previously grown in the
Purification of chondroitinase B and chondroitinase C using glycosaminoglycan-bound AH-Sepharose 4B.
N Ototani et al.
Carbohydrate research, 70(2), 295-306 (1979-05-01)
Chondroitinase B and chondroitinase C were separated from an extract of Flavobacterium heparinum induced with chondroitin 6-sulfate by using column chromatography on hydroxylapatite. Chondroitinase C was eluted together with the activities of hyaluronidase, delta4,5glycosiduronase, and sulfatase. The latter two activities
Artikel
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
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