A6941

Alkohol-Oxidase aus Candida boidinii

lyophilized powder, 5-15 units/mg protein

• Synonym(e): Alcohol:oxygen oxidoreductase
• CAS-Nummer: 9073-63-6
• EC-Nummer: 1.1.3.13 (BRENDA, IUBMB)
• EG-Nummer: 232-971-3
• MDL-Nummer: MFCD00130452
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: fungus (Candida boidinii)
• Qualitätsniveau: 200
• Form: lyophilized powder
• Spezifische Aktivität: 5-15 units/mg protein
• Mol-Gew.: octomer 600 kDa by sedimentation equilibrium
• Löslichkeit: 100 mM potassium phosphate, pH 7.5: soluble 1.0 mg/mL at 25 °C (Cold)
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Research area: Cell Signaling

Alcohol Oxidase (AOX) is a homo-octamer composed of eight flavin adenine dinucleotide (FAD) cofactors and belongs to the glucose-methanol-choline (GMC) family of oxidoreductases. The AOX1 and AOX2 genes are responsible for encoding AOX. Alcohol oxidase is primarily localized in the peroxisome but is also found in the cytoplasm. Alcohol oxidase is a 600 kDa homooctomeric flavoprotein with eight equal 74 kDa subunits; each containing a flavin adenine dinucleotide (FAD) molecule.

Anwendung

Alcohol oxidase has been used:

• to catalyze the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes .
• to study methanol metabolism in yeasts, such as Candida, Pichia, and Hansenula.
• to study protein translocation into peroxisomes.
• for the determination of ethanol concentration in alcoholic drinks using enzymatic assay.
• in development of enzyme electrode for the determination of alcohols.

Biochem./physiol. Wirkung

Alcohol oxidase has the highest affinity for methanol. The affinity decreases with increasing chain length of the alkyl (R) group. The enzyme shows little activity toward secondary, tertiary, or aromatic alcohols; or aliphatic alcohols with a chain length of more than 5 carbons. The pH range for activity of this product is 6.5-8.5, with the optimum pH being 7.5. Alcohol oxidases facilitate the conversion of alcohol into carbonyl compounds while producing hydrogen peroxide. It is also the first enzyme in the yeast methanol utilization pathway.

Einheitendefinition

1 U oxidiert 1.0 μmol Methanol zu Formaldehyd pro Minute bei pH 7.5 und 25°C.

Physikalische Form

Enthält Kaliumphosphat-Puffersalze, DTE und Stabilisator

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)