A2264
β-N-Acetylglucosaminidase aus Canavalia ensiformis (Jack bean)
ammonium sulfate suspension, ≥10 units/mg protein
Synonym(e):
β-N-Acetyl-D-hexosaminid-N-acetylhexosaminohydrolase aus Schwertbohnen, β-N-Acetylhexosaminidase
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About This Item
Empfohlene Produkte
Biologische Quelle
Canavalia ensiformis
Qualitätsniveau
Form
ammonium sulfate suspension
Spezifische Aktivität
≥10 units/mg protein
Fremdaktivität
α- and β-galactosidase, and α-L-fucosidase ≤0.1%
α-mannosidase ≤0.2%
Lagertemp.
2-8°C
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Anwendung
β -N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl-β-D-glucosaminides and N-acetyl-β-Dgalactosaminides. It is used in chemoenzymatic synthesis of oligosaccharides based on their effective transglycosylation of β-GlcNAc and β-GalNAcc. It may be a useful tool to study Alzheimer′s Disease . Acetylglucosaminidase from Canavalia ensiformis has been used to study enzymic detachment of biofilms .
Biochem./physiol. Wirkung
This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. The activity of β-N-actylglucosaminidase may be determined with the chromogenic substrate p-nitrophenyl-N-acetyl-β-D-glucosaminide. β-N-actylglucosaminidase hydrolyzes the terminal nonreducing N-acetyl-D-hexosamine residues. This enzyme contains two predominant isozymes, Hex A, a heterodimer, and Hex B, a homodimer. N-acetylglucosamine, acetamide, N-2-acetamido-2-deoyglucosylamine, N-acetylnojirimycin, and N-acetyldeoxynojirmycin are known inhibitors.
Wird auch als β-N-Acetylhexosaminidase bezeichnet. Es wurde die Freisetzung von terminalem β-verknüpften N-Acetylglucosamin und N-Acetylgalactosamin durch dieses Enzym beobachtet.
Einheitendefinition
1 U hydrolysiert 1.0 μmol p-Nitrophenyl N-acetyl-β-D-glucosaminid zu p-Nitrophenol und N-acetyl-D-glucosamin pro Minute bei pH 5.0 und 25 °C.
Physikalische Form
Suspension in 2.5 M (NH4)2SO4, pH 7.0
Hinweis zur Analyse
Bei pH 4.0 wird p-Nitrophenyl β-N-acetylgalactosaminid mit ca. 50% der Hydrolysegeschwindigkeit von p-Nitrophenyl β-N-acetylglucosaminid bei pH 5.0 hydrolysiert.
Inhibitor
Produkt-Nr.
Beschreibung
Preisangaben
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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Artikel
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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