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Merck

69591

Sigma-Aldrich

4-Methylumbelliferyl α-D-Glucopyranosid

≥98% (TLC)

Synonym(e):

4-Methylumbelliferyl-α-D-glucosid

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About This Item

Empirische Formel (Hill-System):
C16H18O8
CAS-Nummer:
Molekulargewicht:
338.31
Beilstein:
1690776
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
PubChem Substanz-ID:
NACRES:
NA.25

Beschreibung

α-glucosidase substrate

Qualitätsniveau

Assay

≥98% (TLC)

Form

powder

Löslichkeit

DMSO: 50 mg/mL, clear, colorless to faintly yellow

Fluoreszenz

λex 316 nm; λem 375 nm (Reaction product)
λex 317 nm; λem 374 nm (pH9.0)
λex 360 nm; λem 449 nm
λex 365 nm; λem 445 nm in 0.1 M Tris pH 8.0 (α-glucosidase)

Lagertemp.

−20°C

SMILES String

CC1=CC(=O)Oc2cc(O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)ccc12

InChI

1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13-,14+,15-,16+/m1/s1

InChIKey

YUDPTGPSBJVHCN-JZYAIQKZSA-N

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Allgemeine Beschreibung

4-Methylumbelliferyl α-D-glucopyranoside is the fluorogenic substrate of alpha-glucosidase enzyme. 4-Methylumbelliferyl α-D-glucopyranoside, also known as 4MU- α-glc, upon reaction leads to the product 4MU, which emits at the peak of 440nm in the fluorescence spectra.

Anwendung

4-Methylumbelliferyl α-D-glucopyranoside is used as a flurogenic substrate for the identification, characterization and kinetic analysis of α-D-glucosidase(s).4-Methylumbelliferyl α-D-glucopyranoside is used for high throughput screening experiments. It is suitable for the diagnosis of Pompe′s disease in kidney and leucocytes.

Verpackung

Bottomless glass bottle. Contents are inside inserted fused cone.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


Analysenzertifikate (COA)

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

A.Holzapfel-Pschorn et al.
Fresenius Journal of Analytical Chemistry, 327, 521-521 (1987)
Shohei Shigeto et al.
Molecular genetics and metabolism, 103(1), 12-17 (2011-02-16)
The high frequency (3.3-3.9%) of acid α-glucosidase pseudodeficiency, c.[1726G>A; 2065G>A] homozygote (AA homozygote), in Asian populations complicates newborn screening for Pompe disease (glycogen storage disease type II or acid maltase deficiency) on dried blood spots, since AA homozygotes have a
D M Broadhead et al.
Clinical genetics, 13(6), 504-510 (1978-06-01)
The diagnosis of Pompe's disease by the assay of acid alpha-glucosidase in kidney and leucocytes was not previously possible because of the presence of another component which had activity at pH 4.0, but was not deficient in the disease. This
Reporter bacteriophage A511::celB transduces a hyperthermostable glycosidase from Pyrococcus furiosus for rapid and simple detection of viable Listeria cells.
Hagens S, de Wouters T, et al.
Bacteriophages, 1, 143-151 (2011)
Lara W Katzin et al.
Journal of clinical neuromuscular disease, 9(4), 421-431 (2008-06-06)
Pompe disease, or glycogen storage disease type II, is a rare autosomal recessive disorder caused by mutations in the gene that encodes for alpha-glucosidase. Presentation in infancy is associated with respiratory failure, cardiomyopathy, and severe muscle weakness. Juvenile- or adult-onset

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