07985

6-Aminofluoreszein

BioReagent, suitable for fluorescence, ~95% (HPLC)

• Synonym(e): Fluoresceinamin Isomer II
• Empirische Formel (Hill-System): C₂₀H₁₃NO₅
• CAS-Nummer: 51649-83-3
• Molekulargewicht: 347.32
• Beilstein: 51708
• EG-Nummer: 257-334-7
• MDL-Nummer: MFCD00005051
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 329748535
• NACRES: NA.32

Eigenschaften

• Produktlinie: BioReagent
• Qualitätsniveau: 100
• Assay: ~95% (HPLC)
• Form: powder
• Verunreinigungen: ≤5% 5-aminofluorescein
• mp (Schmelzpunkt): 285 °C (dec.) (lit.)
• Löslichkeit: DMSO: soluble; acetone: soluble; methanol: soluble
• Fluoreszenz: λ_ex 490 nm; λ_em 520 nm in 0.1 M Tris pH 9.0
• Eignung: suitable for fluorescence
• SMILES String: Nc1ccc2C(=O)OC3(c4ccc(O)cc4Oc5cc(O)ccc35)c2c1
• InChI: 1S/C20H13NO5/c21-10-1-4-13-16(7-10)20(26-19(13)24)14-5-2-11(22)8-17(14)25-18-9-12(23)3-6-15(18)20/h1-9,22-23H,21H2
• InChIKey: YOAWSYSKQHLFPM-UHFFFAOYSA-N

Beschreibung

Anwendung

6-Aminofluorescein has been demonstrated to be useful fluorescent labeling reagent for fullerene-based liposome nanostructures termed ‘buckysomes′. 6-aminofluorescein dissolved in 0.1 N NaOH can be quantified from a maximum absorbance at 490 nm. Decarboxylated 6-aminofluorescein dissolved 6 N HCl (prepare by constant boiling at 110° C for 24 h) can be quantified from a maximum absorbance at 454 nm. Aldehyde groups are formed by oxidateive damage to proteins, which can be detected by conjugation to decarboxylated 6-aminofluorescein.

Sonstige Hinweise

Nicht zu verwechseln mit Fluorescamin.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Gloves