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Merck

248266

Sigma-Aldrich

Xylidylblau I

synthetic colorimetric reagent

Synonym(e):

4-Hydroxy-3-[2-hydroxy-3-(2,4-dimethylphenylaminocarbonyl)-1-naphthylazo]-benzolsulfonsäure Natriumsalz, Magonsulfonat

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About This Item

Lineare Formel:
HOC6H3[N=NC10H5(OH)[CONHC6H3(CH3)2]]SO3Na
CAS-Nummer:
Molekulargewicht:
513.50
Beilstein:
4107363
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12171500
PubChem Substanz-ID:
NACRES:
NA.47

Qualitätsniveau

Form

powder or crystals

Methode(n)

titration: suitable

Farbe

dark violet

λmax

612 nm

Anwendung(en)

diagnostic assay manufacturing
hematology
histology

Lagertemp.

room temp

SMILES String

[Na+].Cc1ccc(NC(=O)c2cc3ccccc3c(\N=N\c4cc(ccc4O)S([O-])(=O)=O)c2O)c(C)c1

InChI

1S/C25H21N3O6S.Na/c1-14-7-9-20(15(2)11-14)26-25(31)19-12-16-5-3-4-6-18(16)23(24(19)30)28-27-21-13-17(35(32,33)34)8-10-22(21)29;/h3-13,29-30H,1-2H3,(H,26,31)(H,32,33,34);/q;+1/p-1/b28-27+;

InChIKey

LKSHJHJGLORTGD-RXQWRGDBSA-M

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Allgemeine Beschreibung

Xylydil Blue I is a synthetic colorimetric reagent that has been widely used for the spectrophotometric detection of magnesium.

Anwendung

Xylydil Blue I is suitable to determine serum magnesium levels using Mg- Xylydil Blue I complex based method.

Biochem./physiol. Wirkung

Xylydil Blue I forms a purple complex with the magnesium ion in alkaline conditions.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Measurement of magnesium in serum and urine with a random access analyzer by use of a modified xylidyl blue-1 procedure.
D Ratge et al.
Clinica chimica acta; international journal of clinical chemistry, 159(2), 197-203 (1986-09-15)
M J Stone et al.
Clinical chemistry, 42(9), 1474-1477 (1996-09-01)
We have evaluated an enzymatic assay for the determination of Mg in serum, based on the activation of a chemically modified form of isocitrate dehydrogenase by Mg2+. In the presence of potassium isocitrate and NADP+, NADPH, which absorbs light at
[Measurement of magnesium in the human sera with xylidyl-blue I. I. The effect of phosphate ion].
Y Takubo et al.
Rinsho byori. The Japanese journal of clinical pathology, 31(11), 1244-1248 (1983-11-01)
Determination of urine magnesium using the magon dye method on the 'Monarch' centrifugal analyser.
W Davidson et al.
Annals of clinical biochemistry, 27 ( Pt 6), 595-596 (1990-11-01)
M R Wills et al.
Magnesium, 5(5-6), 317-327 (1986-01-01)
A variety of methods have been reported for the quantitative analysis of magnesium in serum and other biological fluids. The methods may be classified into the following groups: magnesium ammonium phosphate; 8-hydroxyquinoline; titan yellow; dihydroxyazobenzene using fluorimetry; Schwarzenbach ethylenediaminetetraacetic acid;

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