11004760001
Roche
Random Primed DNA Labeling Kit
Synonym(e):
nucleic acid labeling
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)
About This Item
UNSPSC-Code:
41105500
Empfohlene Produkte
Verwendung
sufficient for 50 labeling reactions
Qualitätsniveau
Hersteller/Markenname
Roche
Lagertemp.
−20°C
Verwandte Kategorien
Allgemeine Beschreibung
Random primed DNA labeling kit is used for the radioactive and nonradioactive labeling of DNA with modified deoxyribonucleoside triphosphate using random oligonucleotides as primers. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers.
Spezifität
Heat inactivation: Stop the reaction by adding 2 μl 0.2 M EDTA (pH 8.0) and/or by heating to 65 °C for 10 minutes.
Anwendung
Random Primed DNA Labeling Kit has been used to label probe or fragments shorter than 1 kb.
Random Primed DNA Labeling Kit is used to uniformly label plasmid or phage DNA with any [α-32P]-dNTP or modified dNTP. Labeled DNA probes with high specific activity are used in a variety of hybridization techniques including:
- Screening of gene libraries
- Southern, dot and northern blots
- In situ hybridizations
Verpackung
1 kit containing 7 components.
Angaben zur Herstellung
Working solution: Preparation dNTP Stock Mix
To avoid pipetting inaccuracy, due to low volumes, prepare a stock mix of unlabeled dNTPs. Aliquots should be stored at -15 to -25 °C. Avoid repeated freezing and thawing.
Preparation of DIG Stock Mix
To avoid pipetting inaccuracy due to low volumes, prepare a DIG stock mix. To do this, mix digoxigenin-11-dUTP [3 mM] and dTTP (vial 5) 1:1. For each labeling reaction 1.6 μl are used.
To avoid pipetting inaccuracy, due to low volumes, prepare a stock mix of unlabeled dNTPs. Aliquots should be stored at -15 to -25 °C. Avoid repeated freezing and thawing.
- dATP, dGTP, dTTP mixture:
For one labeling reaction pipette:
1 μl dATP
1 μl dGTP
1 μl dTTP to a reaction vial
Preparation of DIG Stock Mix
To avoid pipetting inaccuracy due to low volumes, prepare a DIG stock mix. To do this, mix digoxigenin-11-dUTP [3 mM] and dTTP (vial 5) 1:1. For each labeling reaction 1.6 μl are used.
Sonstige Hinweise
For life science research only. Not for use in diagnostic procedures.
Nur Kit-Komponenten
Produkt-Nr.
Beschreibung
- Control DNA lambda, 20 μl 12.5 µg/ml
- dATP, 50 μl 0.5 mM
- dCTP, 50 μl 0.5 mM
- dGTP, 50 μl 0.5 mM
- dTTP, 50 μl 0.5 mM
- Hexanucleotide mixture in 10x reaction buffer (100 μl)
- Klenow enzyme, labeling grade (100 U)
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 1
Flammpunkt (°F)
does not flash
Flammpunkt (°C)
does not flash
Analysenzertifikate (COA)
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Protokolle
Random Primed DNA Labeling Kit Protocol & Troubleshooting
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