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10108014001
Roche
Papain
from Carica papaya
Synonym(e):
cystein protease, papain
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About This Item
Empfohlene Produkte
Biologische Quelle
papaya
Qualitätsniveau
Form
suspension
Spezifische Aktivität
30 U/mg
~30 units/mg protein (At 25 °C with BAEE as the substrate.)
Mol-Gew.
23 kDa
Verpackung
pkg of 10 mL (100 mg)
Hersteller/Markenname
Roche
Konzentration
10-100 mg/mL
Optimaler pH-Wert
6.0-7.0
Versandbedingung
wet ice
Allgemeine Beschreibung
Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site.
Anwendung
Papain has been used in the digestion of various brain cells.
Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.
Biochem./physiol. Wirkung
Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine. It can catalyze several reactions, such as hydrolysis, transferase action, specificity and acyl-enzyme intermediate.
Leistungsmerkmale und Vorteile
Inhibitors: SH-blocking reagents, iodoacetic acid, iodoacetamide, TPCK, TLCK, leupeptin, a2-macroglobulin, E-64, PMSF, and antipain, Hg2+, and other heavy metals
Physikalische Form
Suspension, crystalline, nonsterile
Angaben zur Herstellung
Working concentration: 0.05 to 0.5 mg/ml
Lagerung und Haltbarkeit
Store at 2 to 8 °C. (a decrease in activity of 20% may occur within 6 months)
Sonstige Hinweise
For life science research only. Not for use in diagnostic procedures.
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
nwg
Flammpunkt (°F)
does not flash
Flammpunkt (°C)
does not flash
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