MABT523
Anti-beta-Actin Antibody, clone RM112
clone RM112, from rabbit
Synonym(e):
Actin, cytoplasmic 1, Beta-actin, beta-Actin
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Alle Fotos(2)
About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
rabbit
Qualitätsniveau
Antikörperform
purified antibody
Antikörper-Produkttyp
primary antibodies
Klon
RM112, monoclonal
Speziesreaktivität
human
Methode(n)
immunocytochemistry: suitable
western blot: suitable
Isotyp
IgG
UniProt-Hinterlegungsnummer
Versandbedingung
wet ice
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... ACTB(60)
Allgemeine Beschreibung
Actin is a 43 kDa highly conserved structural protein. There are three main actin isotypes (alpha, beta and gamma) which show >90% amino-acid (aa) homology between isotypes and >98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. Beta actin is one of the two nonmuscle cytoskeletal actins and is relatively stable and appears to remain at normal levels regardless of experimental treatment. It is generally used as an internal control for experiments.
Immunogen
A peptide corresponding to the N-terminus of beta-Actin.
Anwendung
Immunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected beta-Actin in A431 cells.
This Anti-beta-Actin Antibody, clone RM112 is validated for use in Western Blotting, Immunocytochemistry for the detection of beta-Actin.
Qualität
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected beta-Actin in A431 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected beta-Actin in A431 cell lysate.
Zielbeschreibung
~45 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physikalische Form
Format: Purified
Sonstige Hinweise
Concentration: Please refer to lot specific datasheet.
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