MABF243

Anti-Par-2 Antibody, clone SAM11

clone SAM11, from mouse

• Synonym(e): Proteinase-activated receptor 2, PAR-2, Coagulation factor II receptor-like 1, G-protein coupled receptor 11, Thrombin receptor-like 1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: SAM11, monoclonal
• Speziesreaktivität: mouse, human
• Methode(n): flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG2aκ
• NCBI-Hinterlegungsnummer: NP_005233
• UniProt-Hinterlegungsnummer: P55085
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... F2RL1(2150)

Beschreibung

Allgemeine Beschreibung

The protein named Proteinase-activated receptor 2 (PAR2/PAR-2), or Coagulation factor II receptor-like 1, G-protein coupled receptor 11, Thrombin receptor-like-1 and encoded by the human gene F2RL1/GPR11/PAR2 is a receptor for trypsin and trypsin-like enzymes coupled to G proteins. Its function is mediated through the activation of several signaling pathways including phospholipase C (PLC), intracellular calcium, mitogen-activated protein kinase (MAPK), I-kappaB kinase/NF-kappaB and Rho. Also as part of the innate and adaptive immunity response, PAR2 acts as sensor for proteolytic enzymes generated during infection and works to promote inflammation and the inflammatory response. PAR2 is also involved in vascular and airway tone and in blood pressure regulation and many other G protein coupled receptor response pathways. PAR2 is a multi-pass membrane protein that is widely expressed in most tissues except brain or skeletal muscle.

Immunogen

Epitope: N-terminus

Linear peptide corresponding to the N-terminus of human Par-2.

Anwendung

Research Category
Entzündung & Immunologie

Research Sub Category
Immunoglobuline & Immunologie

Anti-Par-2 Antibody, clone SAM11 is an antibody against Par-2 for use in western blotting, IHC & flow cytometry.

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Par-2 in human small intestine, coronary artery and aorta sections, and in human artery and vein tissues (Molino, M., et al. (1998). Arterioschler Throm Vasc Biol. 18(5):825-832.).

Flow Cytometry Analysis: Arepresentative lot from an independent laboratory detected Par-2 in Par-2 transfected COS-1 cells (Molino, M., et al. (1997). J Biol Chem. 272(17):11133-11141.).

Qualität

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Par-2 in 10 µg of NIH/3T3 cell lysate.

Zielbeschreibung

~50 kDa observed. The calculated MW of this protein is 44 kDa However, it may be observed at higher molecular weights due to glycosylation.

Physikalische Form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable