MABF1991
Anti-Ly9 (CD229) Antibody, clone Ly9.7.144
purified antibody, from mouse
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About This Item
Empfohlene Produkte
Biologische Quelle
mouse
Antikörperform
purified antibody
Klon
Ly9.7.144, monoclonal
Mol-Gew.
calculated mol wt 73.14 kDa
Speziesreaktivität
mouse
Methode(n)
flow cytometry: suitable
immunodepletion: suitable
immunofluorescence: suitable
Isotyp
IgG1κ
Protein-ID-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Lagertemp.
−20°C
Posttranslationale Modifikation Target
unmodified
Spezifität
Clone Ly9.7.144 detects T-lymphocyte surface antigen Ly-9 in murine cells. It targets an epitope with in the N-terminal Ig domain.
Immunogen
300.19 mouse tumor cells transfected with full-length murine Ly9 protein.
Anwendung
Anti-Ly9 (CD229), clone Ly9.7.144, Cat. No. MABF1991, is a mouse monoclonal antibody that detects T-lymphocyte surface antigen Ly-9 and has been tested for use as an Agonist and in Flow Cytometry, Immunofluorescence, Immunodepletion.
Quality Control Testing: Evaluated by Flow Cytometry in Mouse splenocytes.
Flow Cytometry Analysis: 1μg of this antibody detected Ly9 (CD229) in one million Mouse splenocytes.
Tested Applications: Flow Cytometry Analysis: A representative lot detected Ly9 (CD229) in Flow Cytometry applications (Sintes, J., et. al. (2013). J Immunol. 190(1):21-6; Cuenca, M., et. al. (2016). J Immunol. 196(2):726-37).
Agonist Analysis: A representative lot of this antibody induced a significant decrease in the production of IL-4 in wild-type mice. (Sintes, J., et. al. (2013). J Immunol. 190(1):21-6).
Immunodepletion Analysis: A representative lot of this antibody selectively depleted splenic MZ and B1 B cells in wild-type BALB/c mice. (Cuenca, M., et. al. (2016). J Immunol. 196(2):726-37; Cuenca, M., et. al. (2018). Eur J Immunol. 48(1):99-105).
Immunofluorescence Analysis: A representative lot detected Ly9 (CD229) in Immunofluorescence applications (Cuenca, M., et. al. (2016). J Immunol. 196(2):726-37).
Quality Control Testing: Evaluated by Flow Cytometry in Mouse splenocytes.
Flow Cytometry Analysis: 1μg of this antibody detected Ly9 (CD229) in one million Mouse splenocytes.
Tested Applications: Flow Cytometry Analysis: A representative lot detected Ly9 (CD229) in Flow Cytometry applications (Sintes, J., et. al. (2013). J Immunol. 190(1):21-6; Cuenca, M., et. al. (2016). J Immunol. 196(2):726-37).
Agonist Analysis: A representative lot of this antibody induced a significant decrease in the production of IL-4 in wild-type mice. (Sintes, J., et. al. (2013). J Immunol. 190(1):21-6).
Immunodepletion Analysis: A representative lot of this antibody selectively depleted splenic MZ and B1 B cells in wild-type BALB/c mice. (Cuenca, M., et. al. (2016). J Immunol. 196(2):726-37; Cuenca, M., et. al. (2018). Eur J Immunol. 48(1):99-105).
Immunofluorescence Analysis: A representative lot detected Ly9 (CD229) in Immunofluorescence applications (Cuenca, M., et. al. (2016). J Immunol. 196(2):726-37).
Zielbeschreibung
T-lymphocyte surface antigen Ly-9 (UniProt: Q01965; also known as Cell surface molecule Ly-9, Lymphocyte antigen 9, SLAM family member 3, SLAMF3, Signaling lymphocytic activation molecule 3, CD229) is encoded by the Ly9 (also known as Ly-9) gene (Gene ID: 17085) in murine species. Ly-9 is a single-pass type I membrane protein that serves as a homophilic cell surface receptor present on all lymphocytes and is highly expressed on innate like lymphocytes. Its activity is controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Ly-9 plays a negative role in the signaling pathways required for innate-like lymphocyte (iNKT) development in the thymus. It also promotes T-cell differentiation into a helper T-cell Th17 phenotype leading to increased IL-17 secretion. Ly-9 is synthesized with a signal peptide (aa 1-47) that is cleaved off in the mature form. The mature form has an extracellular domain (aa 48-453), a short transmembrane domain (aa 454-474), and a cytoplasmic domain (aa 475-654). The extracellular domain contains two Ig-like V type (aa 48-158 and 250-362) and two Ig-like C type (aa 159-243 and 353-453) domains. In addition, LY-9 has two immunoreceptor tyrosine-based switch motifs (ITSM) motifs (aa 599-604 and 623-628), which mediate the interaction with the SH2 domain of SH2D1A and SH2D1B. Ly-9 deficient mice are reported to develop normally, but exhibit a significant difference in the distribution of thymocyte subsets compared to wild types. This antibody (clone Ly9.7.144) is shown to reduce the number of iNKT cells in the spleen and also reduce the absolute number of both NKT1 and NKT2 cells. (Ref.: Sintes, J., et al. (2013). J. Immunol. 190(1); 21-25; Cuenca, M et al., (2018). Eur. J. Immunol. 48(1); 99 105).
Physikalische Form
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Lagerung und Haltbarkeit
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Sonstige Hinweise
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Analysenzertifikate (COA)
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