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Key Documents

MABE343-AF647

Sigma-Aldrich

Anti-Puromycin, clone 12D10, Alexa Fluor 647 Conjugate Antibody

clone 12D10, 0.5 mg/mL, from mouse

Synonym(e):

Alexa Fluor 647 Anti-Puromycin, Clone 12D10 Anti-Puromycin, Puromycin Detection Antibody

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Konjugat

ALEXA FLUOR 647

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

12D10, monoclonal

Speziesreaktivität

human

Konzentration

0.5 mg/mL

Methode(n)

immunocytochemistry: suitable

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3′ end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
This Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.

Spezifität

Predicted to react with cells from all species when pre-incubated with puromycin.

Qualität

Evaluated by Immunocytochemistry Analysis untreated and puromycin treated HeLa cells.

Immunocytochemistry Analysis: A 1:2,500 dilution of this antibody detected puromycin-incorporated neosynthesized proteins in puromycin treated HeLa cells.

Alexa Fluor is a registered trademark of Life Technologies.

Zielbeschreibung

Refer to Cat. No. MABE343 for observed molecular weight information.

Rechtliche Hinweise

ALEXA FLUOR is a trademark of Life Technologies

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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T cell stimulation is metabolically demanding. To exit quiescence, T cells rely on environmental nutrients, including glucose and the amino acids glutamine, leucine, serine, and arginine. The expression of transporters for these nutrients is tightly regulated and required for T cell activation.
Seema Qamar et al.
Cell, 173(3), 720-734 (2018-04-21)
Reversible phase separation underpins the role of FUS in ribonucleoprotein granules and other membrane-free organelles and is, in part, driven by the intrinsically disordered low-complexity (LC) domain of FUS. Here, we report that cooperative cation-π interactions between tyrosines in the
Noémie Mazaré et al.
Cell reports, 32(8), 108076-108076 (2020-08-28)
Local translation is a conserved mechanism conferring cells the ability to quickly respond to local stimuli. In the brain, it has been recently reported in astrocytes, whose fine processes contact blood vessels and synapses. Yet the specificity and regulation of
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The Journal of experimental medicine, 218(5) (2021-03-17)
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