MAB2115

Anti-Cell Surface Antigen of Human Nucleated Erythroid Cells Antibody, clone HAE9

clone HAE9, Chemicon^®, from mouse

• UNSPSC-Code: 12352203
• eCl@ss: 32160702

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: HAE9, monoclonal
• Speziesreaktivität: human
• Darf nicht reagieren mit: rat, mouse
• Hersteller/Markenname: Chemicon^®
• Methode(n): flow cytometry: suitable; immunoprecipitation (IP): suitable
• Isotyp: IgMκ
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Spezifität

MAB2115 recognizes a 70 kDa surface protein in about 100% nucleated erythroid cells (erythroblasts, normoblasts, proerythroblasts), 36% of late erythroid committed cells and 70-80% of fetal liver cells. Normal stem cells, early erythroid committed cells and hematopoetic non-erythroid cells do not react with MAB2115. The 70 kDa protein may be associated with an isoform of transferrin specific for red cells. Applicable for (1) immunostaining and enumeration of nucleated erythroid cells in bone marrow, fetal liver or cord blood and (2) immunophenotyping human leukemias. {Mechetner, EB et al (1987) Identification of a human erythroid cell surface antigen by monoclonal antibody HAE9. Exp Hematol. 15(4):355-9}.

Immunogen

Human fetal liver cells

Anwendung

Research Category
Entzündung & Immunologie

Research Sub Category
Immunoglobuline & Immunologie

Immunoprecipitation: 5μg/500μL of cell lysate in 0.5% triton X-100 diluted in PBS with proteinase inhibitors

Flow cytometry: 10μg/mL concentration; (1:100); per 10(e6) cells, 100μL sample volume.

Optimal working dilutions must be determined by the end user.

This Anti-Cell Surface Antigen of Human Nucleated Erythroid Cells Antibody, clone HAE9 is validated for use in FC, IP for the detection of Cell Surface Antigen of Human Nucleated Erythroid Cells.

Physikalische Form

Format: Purified

Purified immunoglobulin via gel filtration from tissue culture supernatants in 20mM PB pH 7.6, 250mM NaCL, with 0.1% sodium azide as a preservative.

Lagerung und Haltbarkeit

Maintain at 2-8°C for up to 6 months after date of receipt. The product may contain precipitation and microcentrifugation is recommended before use.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable