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Merck

MAB2000

Sigma-Aldrich

Anti-Integrin β1 Antibody, clone HB1.1

clone HB1.1, Chemicon®, from mouse

Synonym(e):

CD29

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

HB1.1, monoclonal

Speziesreaktivität

human, pig

Hersteller/Markenname

Chemicon®

Methode(n)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotyp

IgG1κ

Eignung

not suitable for activity/function inhibition

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... ITGB1(3688)

Spezifität

Reacts with beta-1 subunit of VLA integrins

Anwendung

Research Category
Zellstruktur
Research Sub Category
Integrine
Anti-Integrin β1 Antibody, clone HB1.1 detects level of Integrin β1 & has been published & validated for use in FC, IP, WB, IH.
Immunoprecipitation of VLA integrins from lysate of 10E6 cell equivalent requires 2 μg of MAB2000.

Flow cytometry: 10 μg/mL

Western blot

Immunohistochemistry in frozen tissue samples: 5 μg/mL

Induction of cell aggregation: 10 μg/mL. MAB2000 is a potent stimulator of cell aggregation tested in U937, Jurkat and human peripheral blood lymphocytes.

MAB2000 does not affect VLA integrin-mediated cell adhesion to matrix proteins.

Optimal working dilutions must be determined by end user.

Verlinkung

Replaces: 04-1109

Physikalische Form

Format: Purified
Purified immunoglobulin from Protein A chromatography. Liquid in 0.02M PB pH 7.6, 0.25M NaCl with 0.1% sodium azide.

Lagerung und Haltbarkeit

Maintain at 2-8°C for up to 6 months after date of receipt.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Empfehlung

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Hypersialylation of beta1 integrins, observed in colon adenocarcinoma, may contribute to cancer progression by up-regulating cell motility.
Seales, EC; Jurado, GA; Brunson, BA; Wakefield, JK; Frost, AR; Bellis, SL
Cancer Research null
PtdIns(3,4,5)P3 regulates spindle orientation in adherent cells.
Toyoshima, F; Matsumura, S; Morimoto, H; Mitsushima, M; Nishida, E
Developmental Cell null
Hyposialylation of integrins stimulates the activity of myeloid fibronectin receptors.
Semel, AC; Seales, EC; Singhal, A; Eklund, EA; Colley, KJ; Bellis, SL
The Journal of Biological Chemistry null
Pig chondrocyte xenoimplants for human chondral defect repair: an in vitro model.
Fuentes-Boquete, Isaac, et al.
Wound Repair and Regeneration, 12, 444-452 (2004)
Mechanical control of cAMP signaling through integrins is mediated by the heterotrimeric Galphas protein.
Francis J Alenghat,Jessica D Tytell,Charles K Thodeti,Alexandrine Derrien,Donald E Ingber
Journal of Cellular Biochemistry null

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