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Merck
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Key Documents

MAB1340

Sigma-Aldrich

Anti-Collagen Type I Antibody, clone C11

ascites fluid, clone C11, Chemicon®

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

100

Antikörperform

ascites fluid

Antikörper-Produkttyp

primary antibodies

Klon

C11, monoclonal

Speziesreaktivität

human

Hersteller/Markenname

Chemicon®

Methode(n)

ELISA: suitable
immunohistochemistry: suitable

Isotyp

IgG

NCBI-Hinterlegungsnummer

NM_000088.3

UniProt-Hinterlegungsnummer

P02452

Versandbedingung

dry ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... COL1A1(1277)

Spezifität

Recognizes both native and heat denatured human collagen type I. No cross reactivity with albumin fractions of human serum or albumins of other animal species. Less than 5% binding to collagens II and II in ELISA, RIA or immunoblotting.

Anwendung

Detect Collagen Type I using this Anti-Collagen Type I Antibody, clone C11 validated for use in ELISA, IH.
Immunohistochemical staining of 10 μm thick cryostat sections fixed with acetone or ethanol. Staining is similar to that for polyclonal antibodies to interstitial collagen I. Suggested working concentration 10-20 μg/mL. Formaldehyde fixation is not recommended. Does not work with Western blots. Not suitable for use on paraffin embedded sections.

ELISA: Apparent binding constant from titration curves is approximately 0.1nM. Antibody suitable for use in sandwich ELISA for specific determination of collagen I, employed as either a capture antibody or as a second, detecting antibody.

Optimal working dilutions must be determined by the end user.

Physikalische Form

Immunoglobulin Purified from ascites by ammonium sulfate precipitation and chromatography on DEAE-cellulose. . Lyophilized, reconstitute with 1 mL of distilled water.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Piktogramme

Health hazardExclamation mark
GHS08,GHS07

Signalwort

Danger

Gefahreneinstufungen

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Lagerklassenschlüssel

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence.
Zoumi, A; Yeh, A; Tromberg, BJ
Proceedings of the National Academy of Sciences of the USA null
Lipoprotein lipase in the arterial wall: linking LDL to the arterial extracellular matrix and much more.
Markku O Pentikainen, Riina Oksjoki, Katariina Oorni, Petri T Kovanen
Arteriosclerosis, Thrombosis, and Vascular Biology null
Functionally distinct human marrow stromal cell lines immortalized by transduction with the human papilloma virus E6/E7 genes.
B A Roecklein, B Torok-Storb
Blood null
Lipoprotein lipase (LPL) strongly links native and oxidized low density lipoprotein particles to decorin-coated collagen. Roles for both dimeric and monomeric forms of LPL.
M O Pentikainen, K Oorni, P T Kovanen
The Journal of Biological Chemistry null
Characterization of the urethral plate and the underlying tissue defined by expression of collagen subtypes and microarchitecture in hypospadias.
Hayashi Y, Mizuno K, Kojima Y, Moritoki Y, Nishio H, Kato T, Kurokawa S, Kamisawa H, Kohri K
International Journal of Urology : Official Journal of the Japanese Urological Association null
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