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Key Documents

MAB1285

Sigma-Aldrich

Anti-HLA-B27 Antibody, clone HLA.ABC.m3

clone HLA.ABC.m3, Chemicon®, from mouse

Synonym(e):

Anti-AS, Anti-B-4901, Anti-HLAB

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

HLA.ABC.m3, monoclonal

Speziesreaktivität

human

Hersteller/Markenname

Chemicon®

Methode(n)

flow cytometry: suitable
immunocytochemistry: suitable

Isotyp

IgG2a

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... HLA-B(3106)

Allgemeine Beschreibung

The previously assigned protein identifier P03989 has been merged into P01889. Full details can be found on the UniProt database.

Spezifität

This antibody binds strongly to the HLA-B27 antigen. It also binds less strongly to the HLA-B7 antigen. Affinity studies by Scatchard analysis showed that the antibody has a higher affinity for HLA-B27 (9.7x108M-1) than for HLA-B7

(9.5x107M-1).

Cell reactivity:

Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:



Subjects Fluorescence Intensity

Heterozygous HLA-B27+ Strong

Homozygous HLA-B7+ Strong

Heterozygous HLA-B7+ Faint

Non B27, Non B7 Negative

Anwendung

This Anti-HLA-B27 Antibody, clone HLA.ABC.m3 is validated for use in FC, IC for the detection of HLA-B27.
This antibody is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis and anterior uveitis.

SUGGESTED USAGE DILUTION

1. Flow cytometry and indirect immunofluorescence 1:60 to 1:120

Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.

2. Cytotoxicity testing 1:25.

Physikalische Form

Format: Purified
The antibody is supplied in phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by flow cytometry.

Lagerung und Haltbarkeit

Store at 2 to 8°C. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Barbara Neerinckx et al.
RMD open, 3(1), e000451-e000451 (2017-09-08)
The strong genetic association between HLA-B27 and ankylosing spondylitis has been known for over 40 years. HLA-B27 positivity is possibly associated with severity of ankylosis. We studied the in vitro and in vivo impact of HLA-B27 in models of chondrogenesis
Description of a mouse monoclonal anti-HLA-B27 antibody HLA-ABC-m3.
Trapani, J A, et al.
Human immunology, 7, 205-216 (1983)

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