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Merck

EZHIL8

Sigma-Aldrich

Human IL-8 ELISA Kit

detect IL-8 in 50 μL serum, plasma or cell culture supernatent

Synonym(e):

Granulocyte chemotactic protein 1, IL-8, Interleukin-8, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, T-cell chemotactic factor

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About This Item

UNSPSC-Code:
41116158
NACRES:
NA.84

product name

Human IL-8 Conferma® ELISA, quantification of Human IL-8 in serum, plasma, or culture media samples

Qualitätsniveau

Speziesreaktivität

human

Parameter

50 μL sample volume (4hr assay)

assay range

accuracy: 77-102 (plasma)%
linearity: 105 (plasma)%

inter-assay cv: <4.7%
intra-assay cv: <4.0%
standard curve range: 0.82-200 pg/mL
accuracy: 70-105 (serum)%
linearity: 94-106 (serum)%

Methode(n)

ELISA: suitable

Aufnahme

sample type plasma (K2 EDTA)
sample type cell culture supernatant
sample type serum

UniProt-Hinterlegungsnummer

Anwendung(en)

research use

Nachweisverfahren

colorimetric (450nm/590nm)

Angaben zum Gen

human ... CXCL8(3576)

Allgemeine Beschreibung

IL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. IL-8 also participates with other cytokines in the proinflammatory signaling cascade. The protein is implicated in numerous pathologies including, bacterial, cancer, cardiovascular and viral diseases.
IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

This is a 96 well format sandwich ELISA which uses our “Conferma” process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots.

Anwendung

This Human Interleukin-8 (IL-8) ELISA kit is used for the quantification of Human IL-8 in serum, plasma, or culture media samples.

Analytes available:
  • IL-8

Research Category:
  • Cytokines
  • Inflammation & Immunology
  • Immune Response
  • Metabolic Disease

Hinweis zur Analyse

3 hour 45 minute assay, room temperature, 50μl of neat serum or plasma is recommened.

Rechtliche Hinweise

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

For research use only. Not for use in diagnostic procedures.

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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