ABC502

Anti-PAR-4 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): PAR-4, PRKC apoptosis WT1 regulator protein, Prostate apoptosis response 4 protein
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): bovine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), canine (based on 100% sequence homology), horse (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• Methode(n): western blot: suitable
• NCBI-Hinterlegungsnummer: NP_002574
• UniProt-Hinterlegungsnummer: Q96IZ0
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PAWR(5074)

Beschreibung

Allgemeine Beschreibung

PAR-4 is a pro-apoptotic protein capable of selectively inducing apoptosis in cancer cells and sensitizing the cells to diverse apoptotic stimuli causing tumor regression. PAR-4 also seems to be a transcriptional repressor and is involved in regulating APP expression and the cleavage activity of BACE1. PAR-4 interacts with several proteins including WT1, but also various kinases, and structural proteins like Actin, BACE1, SPSB1, SPSB2, and SPSB4. PAR4 is localized in the cytoplasm in non-apoptotic cells but is nuclear localized in cancer and apoptotic cells. PAR-4 is widely expressed and elevated in neurodegenerative diseases such as ALS, Alzheimer, Parkinson and Huntington diseases as well as stroke. PAR-4 is down regulated in cancers.

Immunogen

Epitope: C-terminus

GST-tagged recombinant protein corresponding to the C-terminus of human PAR-4.

Anwendung

Research Category
Apoptose & Krebs

Research Sub Category
Apoptose - Weiterführende Produkte

Anti-PAR-4 Antibody is an antibody against PAR-4 for use in Western Blotting.

Qualität

Evaluated by Western Blotting in Y-79 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected PAR-4 in 10 µg of Y-79 cell lysate.

Zielbeschreibung

~45 kDa observed. The calculated molecular weight is 36 kDa, however PAR-4 has been shown as a ~45 kDa band in some western blots (Masters, S.L., et al. (2006). Nature Structural & Mole. Biol. 13(1):77-84).

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable