AB2290

Anti-MAP-2 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): microtubule-associated protein 2, MAP2, MAP 2
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: rat
• Methode(n): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI-Hinterlegungsnummer: NP_037198
• UniProt-Hinterlegungsnummer: P15146
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Microtubule-associated protein 2 (UniProt: P15146; also known as MAP-2) is encoded by the Map2 (also known as Mtap2) gene (Gene ID: 25595) in rat. MAP-2 belong to the family of thermostable proteins associated with microtubules. It is an abundant neuronal cytoskeletal protein that binds to tubulin and stabilizes microtubules against depolymerization. It also has a stiffening effect on microtubules. MAP-2 is shown to be essential for the development and maintenance of neuronal morphology. Three primary isoforms of MAP-2, the high molecular weight MAP2a and MAP2b, and the low molecular weight MAP2c (resulting from alternate splicing of the MAP2 gene) have been identified in neurons. The low molecular weight isoform, MAP2c, is expressed in developing brain and is down-regulated during brain maturation, whereas the high molecular weight MAP2b is expressed in both developing and adult brain. The MAP2a appears only after brain maturation. All these forms bind to microtubules through a domain near the carboxyl terminus that contains either three or four similar repeats of a 31-amino-acid motif. MAP-2 can be phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2) that causes its detachment from microtubules and results in their disassembly.

Spezifität

Other homologies: Mouse (94% sequence homology). Human (72% sequence homology).

This antibody recognizes MAP-2.

Immunogen

Three KLH-conjugated linear peptides corresponding to the N-terminal and central regions of rat MAP-2 .

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Neuronen- & Gliamarker

Anti-MAP-2 Antibody, Cat. No. AB2290, is a highly specific rabbit polyclonal antibody that targets rat MAP-2 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected MAP-2 in E18 rat hippocampus neurons.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MAP-2 in mouse cerebellum, rat cerebral cortex, and rat kidney tissues.

Qualität

Evaluated by Western Blotting in rat brain microsomal preparation.

Western Blotting Analysis: 1 µg/mL of this antibody detected MAP-2 in 10 µg of rat brain microsomal preparation.

Zielbeschreibung

~270 kDa observed; 202.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
Rat brain microsomal tissue lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable