8.05740

2-Mercaptoethanol

for synthesis

• Synonym(e): Hydroxyethylmercaptan, Thioethylenglycol, Thioglycol, β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylenglycol
• Lineare Formel: HSCH₂CH₂OH
• CAS-Nummer: 60-24-2
• Molekulargewicht: 78.13
• Beilstein: 773648
• MDL-Nummer: MFCD00004890
• UNSPSC-Code: 12352104
• EG-Index-Nummer: 200-464-6
• NACRES: NA.22

Eigenschaften

• Dampfdichte: 2.69 (vs air)
• Qualitätsniveau: 200
• Dampfdruck: 1 mmHg ( 20 °C)
• Assay: ≥99% (GC)
• Form: liquid
• Selbstzündungstemp.: 295 °C
• Wirksamkeit: 98-162 mg/kg LD₅₀, oral (Rat); 112-224 mg/kg LD₅₀, skin (Rabbit)
• Expl.-Gr.: 18 %
• Eignung der Reaktion: reagent type: reductant
• Konzentration: 14.3 M (pure liquid)
• Brechungsindex: n20/D 1.500 (lit.)
• pH-Wert: 4.5-6 (20 °C, 500 g/L in H₂O)
• bp: 157 °C (lit.)
• mp (Schmelzpunkt): <-50 °C
• Übergangstemp.: flash point 70.5 °C
• Dichte: 1.114 g/mL at 25 °C (lit.)
• Lagertemp.: 2-30°C
• SMILES String: OCCS
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• InChIKey: DGVVWUTYPXICAM-UHFFFAOYSA-N

Beschreibung

Anwendung

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Sicherheitshinweise

• Piktogramme: GHS05,GHS06,GHS08,GHS09
• Signalwort: Danger
• H-Sätze: H301 + H331 - H310 - H315 - H317 - H318 - H361d - H373 - H410
• P-Sätze: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
• Lagerklassenschlüssel: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flammpunkt (°F): 165.2 °F - closed cup
• Flammpunkt (°C): 74 °C - closed cup