676604

PhosphoDetect Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6)

lyophilized, clone 5C6, Calbiochem^®

• Synonym(e): Anti-Vasodilator-stimulated Phosphoprotein, Activated
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 5C6, monoclonal
• Form: lyophilized
• Enthält: ≤0.1% sodium azide as preservative
• Speziesreaktivität: human, mouse
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Isotyp: IgG1
• Versandbedingung: ambient
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: phosphorylation (pSer157)
• Angaben zum Gen: human ... VASP(7408)

Beschreibung

Allgemeine Beschreibung

Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the the ~50 kDa VASP protein phosphorylated at Ser¹⁵⁷.

Recognizes the the ~50 kDa VASP protein phosphorylated at Ser¹⁵⁷.

This PhosphoDetect Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer¹⁵⁷).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP

Anwendung

ELISA (0.05 µg/ml)

Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)

Immunocytochemistry (1-10 µg/ml)

Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

Lyophilized from PBS, PEG, sucrose.

Rekonstituierung

Reconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.

Hinweis zur Analyse

Positive Control
Vanadate-treated A431 cells

Sonstige Hinweise

Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol.13, 158.
Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol.166, 5550.
Burkhardt, M., et al. 2000. J. Biol. Chem.275, 33536.
Harbeck, B., et al. 2000. J. Biol. Chem.275, 40.
Smolenski, A., et al. 2000. J. Biol. Chem.275, 25723.

Does not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable