5.34329

H₂S Fluorescent Probe, P3

• Synonym(e): H₂S Fluorescent Probe, P3, ( E)-2-(3-(6-(2-Hydroxyethylamino)naphthalen-2-yl)-3-oxoprop-1-enyl)-3,5-dimethoxybenzaldehyde, Hydrogen Sulfide Fluorescent Probe, P3, Probe P3, (E)-2-(3-(6-(2-Hydroxyethylamino)naphthalen-2-yl)-3-oxoprop-1-enyl)-3,5-dimethoxybenzaldehyde, Hydrogen Sulfide Fluorescent Probe, P3, Probe P3
• Empirische Formel (Hill-System): C₂₄H₂₃NO₅
• Molekulargewicht: 405.44
• UNSPSC-Code: 12352202
• NACRES: NA.28

Eigenschaften

• Assay: ≥98% (HPLC)
• Qualitätsniveau: 100
• Form: solid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; protect from light
• Farbe: orange
• Löslichkeit: DMSO: 100 mg/mL
• Fluoreszenz: λ_ex 375 nm; λ_em 505 nm
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

A cell-permeable, non-toxic, non-fluorescent naphthalenyl-dimethoxybenzaldehyde enone compound that acts as a highly specific, sensitive, fast-responsive H₂S fluoroprobe (signal saturation at ≤ 5 min with a detection limit of 50 nM). Dose-dependently detects hydrogen sulfide levels under physiological conditions and readily fluoresces. Displays little to no reactivity towards GSH, Cys, H₂O₂, citrate and iodide (Hepes pH 7.4, containing 10% FBS). Also shown to be useful as a two-photon probe for imaging endogeneous H₂S levels in HeLa cells. A thiol specific fluorescent probe is also available (Cat. No. 595504).

A cell-permeable, non-toxic, non-fluorescent naphthalenyl-dimethoxybenzaldehyde enone compound that acts as a highly specific, sensitive, fast-responsive H₂S fluoroprobe (signal saturation at ≤ 5 min with a detection limit of 50 nM). Dose-dependently detects hydrogen sulfide levels under physiological conditions and readily fluoresces. Displays little to no reactivity towards GSH, Cys, H₂O₂, citrate and iodide (Hepes pH 7.4, containing 10% FBS). Also shown to be useful as a two-photon probe for imaging endogeneous H₂S levels in HeLa cells. A thiol specific fluorescent probe is also available (Cat. No. 595504).

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

Biochem./physiol. Wirkung

Cell permeable: yes

Primary Target
hydrogen sulfide

Reversible: no

Verpackung

Packaged under inert gas

Warnhinweis

Toxicity: Regulatory Review (Z)

Rekonstituierung

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sonstige Hinweise

Singha, S., et al. 2015. Anal. Chem.87, 1188.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable