513001

PD 98059

InSolution, ≥98%, 5 mg/ml, reversible inhibitor of MAP Kinase Kinase (MEK)

• Synonym(e): InSolution PD 98059, MEK Inhibitor V
• Empirische Formel (Hill-System): C₁₆H₁₃NO₃
• CAS-Nummer: 167869-21-8
• Molekulargewicht: 267.28
• MDL-Nummer: MFCD00671789
• UNSPSC-Code: 12352200
• NACRES: NA.77

Eigenschaften

• Qualitätsniveau: 100
• Assay: ≥98% (HPLC)
• Form: liquid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; protect from light
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• InChI: 1S/C16H13NO3/c1-19-14-8-4-6-11(16(14)17)15-9-12(18)10-5-2-3-7-13(10)20-15/h2-9H,17H2,1H3
• InChIKey: QFWCYNPOPKQOKV-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Selective and cell-permeable inhibitor of MAP kinase kinase (MEK) that acts by inhibiting the activation of MAP kinase and subsequent phosphorylation of MAP kinase substrates. Pretreatment of PC-12 cells with PD 98059 completely blocks the 4-fold increase of MAP kinase activity produced by nerve growth factor (NGF; IC₅₀ = 2 µM); however, it has no effect on NGF-dependent tyrosine phosphorylation of the pp140^trk receptor or its substrate Shc and does not block NGF-dependent activation of PI 3-kinase. Inhibits cell growth and reverses the phenotype of ras-transformed BALB 3T3 mouse fibroblasts and rat kidney cells.

Biochem./physiol. Wirkung

Cell permeable: yes

Primary Target
MAP kinase kinase (MEK)

Product does not compete with ATP.

Reversible: yes

Target IC₅₀: 2 µM in completely blocking the 4-fold increase in MAP kinase activity produced by nerve growth factor in PC12 cells

Verpackung

Packaged under inert gas

Warnhinweis

Toxicity: Irritant (B)

Physikalische Form

A 5 mg/ml solution of PD 98059 (Cat. No. 513000) in anhydrous DMSO.

Rekonstituierung

Following initial thaw, aliquot and freeze.

Sonstige Hinweise

Kültz, D., et al. 1998. J. Biol. Chem.273, 13645.
Dudley, D.T., et al. 1995. Proc. Natl. Acad. Sci. USA92, 7686.
Langlois, W.J., et al. 1995. J. Biol. Chem.270, 25320.
Pang, L., et al. 1995. J. Biol. Chem.270, 13585.
Waters, S.B., et al. 1995. J. Biol. Chem.270, 20883.



Selected Citations
Lee, J., et al. 2009. Cell Stem Cell5, 76.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Flammpunkt (°C): 87 °C - closed cup - (Dimethylsulfoxide)