405210

ID-8

A cell-permeable indole compound that, when added to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF

• Synonym(e): ID-8, 1-(4-Methoxyphenyl)-2-methyl-3-nitro-indol-6-ol
• Empirische Formel (Hill-System): C₁₆H₁₄N₂O₄
• CAS-Nummer: 147591-46-6
• Molekulargewicht: 298.29
• MDL-Nummer: MFCD00593367
• UNSPSC-Code: 12352200
• NACRES: NA.51

Eigenschaften

• Qualitätsniveau: 100
• Assay: ≥95% (HPLC)
• Form: powder
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; protect from light
• Farbe: yellow
• Löslichkeit: DMSO: 50 mg/mL
• Versandbedingung: ambient
• Lagertemp.: 2-8°C
• SMILES String: [N+](=O)([O-])c1c2c([n](c1C)c3ccc(cc3)OC)cc(cc2)O
• InChI: 1S/C16H14N2O4/c1-10-16(18(20)21)14-8-5-12(19)9-15(14)17(10)11-3-6-13(22-2)7-4-11/h3-9,19H,1-2H3
• InChIKey: VVZNWYXIOADGSW-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

A cell-permeable indole compound that, when added (10 µM) to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF (1,000 units/ml) for expanding ESCs in their undifferentiated state. Long-term murine ESCs maintained under ID-8 are capable of differentiating and EBs (Embryoid Bodies) formation upon ID-8 removal. Also claimed to maintain human ESCs in their pluripotent state in the absence of MEF.

Verpackung

Packaged under inert gas

Warnhinweis

Toxicity: Standard Handling (A)

Sonstige Hinweise

Miyabayashi, T., et al. 2008. Biosci. Biotechnol. Biochem.72, 1242.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3