298711

Diethyl Pyrocarbonate

Inhibits ryanodine binding to ryanodine/Ca²⁺ receptor channel in skeletal muscle in a dose- and time-dependent manner and increases the Ca²⁺ permeability of SR vesicles.

• Synonym(e): Diethyl Pyrocarbonate, Diethyl Oxydiformate, DEPC
• Empirische Formel (Hill-System): C₆H₁₀O₅
• CAS-Nummer: 1609-47-8
• Molekulargewicht: 162.14
• MDL-Nummer: MFCD00009106
• UNSPSC-Code: 12352200
• NACRES: NA.77

Eigenschaften

• Qualitätsniveau: 100
• Assay: ≥97% (titration)
• Form: liquid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; protect from light
• Farbe: clear colorless
• Löslichkeit: chloroform: soluble; ethanol: soluble
• Versandbedingung: ambient
• Lagertemp.: 2-8°C
• InChI: 1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
• InChIKey: FFYPMLJYZAEMQB-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Inhibits ryanodine binding to ryanodine/Ca²⁺ receptor channel in skeletal muscle in a dose- and time-dependent manner and increases the Ca²⁺ permeability of SR vesicles. Useful for specific inactivation of nucleases during isolation of undegraded polynucleotides. Also inhibits platelet-activating factor acetyl hydrolase. Has been used as a probe for the topography of 5.8S rRNA in yeast ribosomes.

Inhibits ryanodine binding to ryanodine/Ca²⁺ receptor channel in skeletal muscle. Useful for specific inactivation of nucleases during isolation of undegraded polynucleotides. Also inhibits platelet-activating factor acetyl hydrolase. Has been used as a probe for the topography of 5.8S rRNA in yeast ribosomes.

Biochem./physiol. Wirkung

Cell permeable: no

Primary Target
Inhibits ryanodine binding to ryanodine/Ca2+ receptor channel in skeletal muscle

Product does not compete with ATP.

Reversible: no

Warnhinweis

Toxicity: Harmful (C)

Sonstige Hinweise

Shoshan-Barmatz, V., and Weil, S. 1994. Biochem. J. 299, 177.
Blumbers, D.O. 1987. Methods Enzymol. 152, 20.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H302
• P-Sätze: P264 - P270 - P301 + P312 - P501
• Gefahreneinstufungen: Acute Tox. 4 Oral
• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): 156.2 °F - closed cup
• Flammpunkt (°C): 69 °C - closed cup