234409

Complement C9-Depleted Serum, Human

• UNSPSC-Code: 12352202
• NACRES: NA.25

Eigenschaften

• Form: liquid
• Enthält nicht: preservative
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Versandbedingung: dry ice
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

Human serum depleted of complement component C9 by affinity chromatography.

Human serum depleted of complement component C9 by affinity chromatography. C9-depleted serum, sterile-filtered through a 0.22 µm filter. No C9 detected by Ouchterlony using various dilutions of goat anti-human C9 antibody.

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Einheitendefinition

Classical Pathway Activity: One C9H50 unit measured by classical pathway activation is defined as the amount of C9 required to yield 50% lysis of 3 x 10⁷EA when incubated in the presence of the recommended volume of C9-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++.CH50 Functional Activity upon Reconstitution: One CH50 unit is defined as the input of C9-Dpl, reconstituted with C9, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++.Alternative Pathway Activity: One unit of whole alternative pathway activity (APH50) is defined as the input of C9-Dpl, reconstituted with C9, or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 µl GVB° containing a final Mg-EGTA concentration of 13.3 mM.

Physikalische Form

In 150 mM NaCl, 50 mM sodium phosphate, pH 7.2 ± 0.2.

Angaben zur Herstellung

Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.

Rekonstituierung

Following initial thaw, aliquot and freeze (-70°C).

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable