208750

ALLN

InSolution, ≥95%, Inhibitor of calpain I, calpain II, cathepsin B, and cathepsin L

• Synonym(e): InSolution ALLN, Calpain Inhibitor I, LLNL, MG 101, Proteasome Inhibitor V
• Empirische Formel (Hill-System): C₂₀H₃₇N₃O₄
• CAS-Nummer: 110044-82-1
• Molekulargewicht: 383.53
• MDL-Nummer: MFCD00065505
• UNSPSC-Code: 12352200
• NACRES: NA.77

Eigenschaften

• Qualitätsniveau: 100
• Assay: ≥95% (HPLC)
• Form: solution
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; protect from light
• Farbe: clear colorless
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• InChI: 1S/C20H37N3O4/c1-7-8-9-16(12-24)22-19(26)18(11-14(4)5)23-20(27)17(10-13(2)3)21-15(6)25/h12-14,16-18H,7-11H2,1-6H3,(H,21,25)(H,22,26)(H,23,27)/t16-,17-,18-/m0/s1
• InChIKey: FMYKJLXRRQTBOR-BZSNNMDCSA-N

Beschreibung

Allgemeine Beschreibung

A 10 mM solution of ALLN (Cat. No. 208719) in anhydrous DMSO. Inhibitor of calpain I (K_i = 190 nM), calpain II (K_i = 220 nM), cathepsin B (K_i = 150 nM), and cathepsin L (K_i = 0.5 nM). Inhibits neutral cysteine proteases and proteasome (K_i = 6 µM). Protects against neuronal damage caused by hypoxia and ischemia. Inhibits apoptosis in thymocytes and metamyelocytes. Inhibits cell cycle progression at the G₁/S border and metaphase/anaphase in CHO cells by inhibiting cyclin B degradation. Also prevents nitric oxide production by activated macrophages by interfering with transcription of the inducible nitric oxide synthase gene. Inhibits the proteolysis of IkB-α and IkB-β by the ubiquitin-proteasome complex.

Biochem./physiol. Wirkung

Cell permeable: yes

Primary Target
calpain 1

Product does not compete with ATP.

Reversible: no

Target K_i: 190 nM, 220 nM, 150 nM, 500 pM, against calpain I, calpain II, cathepsin B, and cathepsin L, respectively

Verpackung

Packaged under inert gas

Warnhinweis

Toxicity: Flammable (J)

Physikalische Form

A 10 mM (5 mg/1.30 ml) solution of ALLN (Cat. No. 208719) in DMSO.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Sonstige Hinweise

Milligan, S.A., et al. 1996. Arch. Biochem. Biophys.335, 388.
Griscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun.215, 721.
Squier, M.K., et al. 1994. J. Cell Physiol.159, 229.
Rami, J., and Kreiglstein, J. 1993. Brain Res.609, 67.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): 188.6 °F - closed cup
• Flammpunkt (°C): 87 °C - closed cup