164598

Aminopeptidase M, Porcine Kidney

• Synonym(e): Aminopeptidase M, Porcine Kidney, Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N
• CAS-Nummer: 9054-63-1
• EC-Nummer: 3.4.11.2 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00131501
• UNSPSC-Code: 12352202

Eigenschaften

• Biologische Quelle: Porcine kidney
• Qualitätsniveau: 100
• Form: suspension (crystalline)
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Farbe: white
• Löslichkeit: physiological buffer: soluble; water: soluble
• Fremdaktivität: Oxidases ≤3%
• Versandbedingung: ambient
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Aminopeptidase M (APM) is abundantly found in brush border membranes of the kidney, small intestine, placenta, and liver. It is a type II integral membrane protein, known as ectoenzyme.

Anwendung

Aminopeptidase M, porcine kidney has been used:

• to test and identify the peaks containing d-residue in the allatotropin-related peptide (ATRP)
• to study its oxidative inactivation of enzyme activity, and to screen MIRA ingredients
• for the proteolysis of isolevuglandins (IsoLGs) proteins

Biochem./physiol. Wirkung

Aminopeptidase M (APM) is implicated in terminating the actions of certain neuropeptides, like the enkephalins and angiotensin III in the brain. It also metabolizes specific vasoactive peptides. It functions as a receptor for certain viruses, such as coronavirus 229E. APM is a nonspecific peptidase, which rapidly breaks down l-residue peptides.

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Harmful (C)

Einheitendefinition

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of Leu-pNA per min at 37°C, pH 7.2.

Physikalische Form

Crystalline suspension in 3.2 M (NH₄)₂SO₄, 10 mM MgCl₂, 10 mM Tris-HCl, pH 7.5.

Sonstige Hinweise

Wachsmuth, E.E., et al. 1966. Biochemistry5, 169.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable