14-475-M

Cdk2/Cyclin E Protein, active, 10 µg

Active complex of recombinant full length human Cdk2 containing a C-terminal His6-tag & recombinant full-length human Cyclin E containing an N-terminal GST-tag. For use in Kinase Assays.

• UNSPSC-Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 100
• Rekombinant: expressed in baculovirus infected Sf21 cells
• Haltbarkeit: 1 yr
• Mol-Gew.: M_w 34 kDa (cdk2); M_w 74 kDa (cyclin)
• Hersteller/Markenname: Upstate^®
• Methode(n): activity assay: suitable (kinase)
• Löslichkeit: water: soluble
• NCBI-Hinterlegungsnummer: NM_001798.2; NM_052827.1
• UniProt-Hinterlegungsnummer: P24941
• Angaben zum Gen: human ... CCNE1(898) , CDK2(898)

Beschreibung

Allgemeine Beschreibung

Research area: APOPTOSIS

Complex of recombinant full length human cdk2 containing a C-terminal His6-tag, and recombinant full-length human cyclin E containing an N-terminal GST-tag. Product Source: Both are expressed by baculovirus in Sf21 insect cells The Cyclin E genes (CCNE1) gene located at 19q12 region in humans is responsible for encoding full-length protein of 410 amino acids.

Biochem./physiol. Wirkung

Protein Target: CDK2/cyclinE

Target Sub-Family: CMGC

The Cyclin E1 protein binds to and activates Cyclin-dependent kinase 2 (CDK2), forming an active complex crucial for the initiation and progression of the S phase. Cyclin E/CDK2 complex primarily regulates cell cycle advancement and DNA replication by phosphorylating specific substrates. Elevated Cyclin E protein levels and heightened CDK2 kinase activity are both individually linked to unfavorable prognosis, decreased survival, and resistance to therapy in cancer patients. Overactivation of the Cyclin E/CDK2 complex directly disrupts DNA replication through multiple mechanisms, resulting in DNA double strand breaks (DSBs) and genomic instability. Targeting the oncogenic Cyclin E/CDK2 complex has emerged as a potential therapeutic approach against cancer.

Verpackung

Also available in 250μg size as catalog # 14-475M - Call for pricing and availability.

Qualität

routinely evaluated by phosphorylation of histone H1 by cdk2/cyclin E

Physikalische Form

Ni-NTA agarose

Lagerung und Haltbarkeit

1year at -70C

Sonstige Hinweise

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.