14-451-M

ROKα/ROCK-II Protein, active, 10 µg

Active, N-terminal His6-tagged recombinant human ROCK-II residues 11-552, for use in Kinase Assays.

• Synonym(e): ROK, Renal carcinoma antigen NY-REN-35, Rho kinase, Rho-associated, coiled-coil-containing protein kinase 1, p160ROCK
• UNSPSC-Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 100
• Rekombinant: expressed in baculovirus infected Sf21 cells
• Form: liquid
• Haltbarkeit: 6 mo.
• Mol-Gew.: M_w 63.3 kDa
• Hersteller/Markenname: Upstate^®
• Methode(n): activity assay: suitable (kinase)
• Löslichkeit: soluble
• NCBI-Hinterlegungsnummer: NM_005406.2
• UniProt-Hinterlegungsnummer: Q13464
• Lagertemp.: −70°C
• Angaben zum Gen: human ... ROCK1(6093)

Beschreibung

Allgemeine Beschreibung

Research area: APOPTOSIS

N-terminal His6-tagged recombinant human ROCK-II residues 11-552
Product Source: expressed by baculovirus in Sf21 insect cells

Rho kinase (ROCK) is a type of serine/threonine kinase. There are two isoforms, ROCK1 and ROCK2. In humans ROCK1 and ROCK2 are composed of 33 exons and located on chromosome 18 (18q11.1). The ROCK protein structure is characterized by a catalytic kinase domain and Rho-binding domain (RBD) at N-terminal and pleckstrin-homology (PH) domain and an internal cysteine-rich (CR) domain at C-terminal.

Biochem./physiol. Wirkung

Protein Target: ROKα/ROCK-II
Target Sub-Family: AGC

ROCK (Rho kinase) is activated by interacting with Rho GTPase enzyme. ROCK plays a crucial role in various essential cellular functions like adhesion, contraction, migration, and proliferation. Moreover, it regulates the cytoskeleton and actomyosin contractility during apoptosis. Overexpression of ROCK protein has been observed in various types of cancer such as osteosarcoma, hepatocellular carcinomas, colon and bladder cancers.

Verpackung

Also available in 250μg size (14-451M), please inquire for pricing and availability.

Qualität

routinely evaluated by phosphorylation of the Long S6 Kinase substrate peptide

Physikalische Form

Ni²⁺/NTA-agarose

Lagerung und Haltbarkeit

6 months at -70°C

Sonstige Hinweise

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.