05-1923

Anti-phospho-Threonine Antibody, clone 20H6.1

clone 20H6.1, from mouse

• Synonym(e): Threonine
• UNSPSC-Code: 12352203
• eCl@ss: 32160702

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörper-Produkttyp: primary antibodies
• Klon: 20H6.1, monoclonal
• Speziesreaktivität: mouse, human
• Speziesreaktivität (Voraussage durch Homologie): vertebrates (based on 100% sequence homology), all
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• Isotyp: IgG2bκ
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: phosphorylation (pThr)

Beschreibung

Allgemeine Beschreibung

Protein phosphorylation is normally associated with protein modification in signaling pathways and may be useful in pathophysiology and treatment of major neuropsychiatric disorders. Antibodies against phospho-threonine have been used to establish stability of the phosphorylated forms of proteins that may be involved in many different types of signaling. Phospho-threonine antibody is used to measure phosphorylation levels of PSII reaction center proteins D1, D3 and chlorophyll a binding protein CP43.

Immunogen

KLH-conjugated linear peptide corresponding to Threonine.

Anwendung

Research Category
Zelluläre Signaltransduktion

Research Sub Category
Allgemeine Posttranslationsmodifikation

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Threonine from 100 µg of A431 cells stimulated with calyculin/okadaic acid.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Threonine in A431, NIH/3T3, and HeLa cells.

This phospho-Threonine antibody is validated for use in WB & ICC for the detection of the phospho-Threonine protein.

Qualität

Evaluated by Western Blot in calyculin/okadaic acid treated and untreated A431 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Threonine on 10 µg of calyculin/okadaic acid treated and untreated A431 cell lysates.

Zielbeschreibung

Various observed

Physikalische Form

Protein G

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
Calyculin/okadaic acid treated and untreated A431 cell lysates.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable