04-119-S

Anti-acetyl-Histone H4 (Lys12) Antibody, Trial Size, rabbit monoclonal

culture supernatant, Chemicon^®

• Synonym(e): H4K12Ac, Histone H4 (acetyl K12)
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: culture supernatant
• Antikörper-Produkttyp: primary antibodies
• Klon: monoclonal
• Speziesreaktivität: human
• Hersteller/Markenname: Chemicon^®
• Methode(n): ChIP: suitable; dot blot: suitable; western blot: suitable
• Isotyp: IgG
• NCBI-Hinterlegungsnummer: NM_003538.3
• UniProt-Hinterlegungsnummer: P62805
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: acetylation (Lys12)
• Angaben zum Gen: human ... H4C1(8359)

Beschreibung

Spezifität

Recognizes Histone H4 when acetylated on Lys12.

Wide range of cross-reactivity expected based on sequence homology.

Immunogen

Peptide corresponding to Histone H4 containing the sequence [GLG-AcK-GGA] on which Lys12 is acetylated.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Histone

Chromatin-Biologie (ChIP)

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µL of Negative Control Supernatant, or 2 µL of Anti-Acetyl-Histone H4 (Lys12) and the Magna ChIP A Kit (Cat. # 17-610).
Successful immunoprecipitation of Acetyl-Histone H4 (Lys12)-associated DNA fragments was verified by qPCR using ChIP Primers, human GAPDH Coding Region as a positive locus, and a gene desert region as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Lysates from HeLa cells untreated or sodium butyrate treated (Lanes 1 and 2 respectively) were resolved probed with anti-acetyl-Histone H4 (Lys12) (1:1,000). Arrow indicates Acetyl-Histone H4 (Lys12).
Arrow indicates Acetyl-Histone H4 (Lys12) (~11 kDa)

This Anti-acetyl-Histone H4 (Lys12) Antibody, Trial Size / Pack is validated for use in WB for the detection of acetyl-Histone H4 (Lys12).

Qualität

Routinely evaluated by immunoblot.

Zielbeschreibung

11kDa

Physikalische Form

10 μL of rabbit monoclonal IgG cell culture supernatant with 0.1% sodium azide.

Lagerung und Haltbarkeit

2 years at -20°C from date of shipment

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable