03-0191-00

SMC^® Human IFN-β1 High Sensitivity Immunoassay Kit

• Synonym(e): Interferon β-1 immunoassay kit, Interferon beta-1 Single Molecule Counting Kit, Interferon beta-1 immunoassay kit, Interferon beta-1 ultra-sensitive immunoassay
• UNSPSC-Code: 12161503
• NACRES: NA.84

Eigenschaften

• Hersteller/Markenname: SMC^®
• assay range: sensitivity: 0.15 pg/mL; (lower limit of quantification); inter-assay cv: 13%; intra-assay cv: 6%
• Methode(n): ELISA: suitable; single molecule counting: suitable
• Relevante Krankheit(en): infectious diseases; autoimmune diseases; neurodegenerative disorders
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

A member of the type I interferon family of glycoprotein second messengers, human Interferon Beta (IFN-ß1) has been associated with antiviral activity as a secreted factor from infected cells that mediates innate immunity in neighboring cells. In addition, IFN-β1 has also been utilized as treatment for Multiple Sclerosis (MS) due to its ability to ameliorate inflammation in the central nervous system in this disease.

The SMC^® Human IFN-β1 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO^® immunoassay systems detect analytes using Single Molecule Counting (SMC^®) technology.

Spezifität

Cross Reactivity:
Cross-reactivity to the following analytes were tested with the following results:

• human IFN-α2 – 0.2%
• human IFN-γ – 0.3%
• human IL-29 (IFN-λ1) – 0.2%
• human IL-28A (IFN-λ2) – 0.2%
• human IL-28B (IFN-λ3) – 0.3%
• human IFN-ω – 0.2%

Accuracy:

• Minimum of 5 serum samples spiked with average recovery of 88% with a range of 78-100%
• Minimum of 5 plasma samples spiked with average recovery of 93% with a range of 75-113%

Anwendung

Research Category:

• Inflammation & Immunology
• Immune Response
• Infectious Diseases
• Signaling
• Neuroscience

Research Sub-Category:

• Inflammatory Disease
• Immune Response
• Autoimmune disease
• Neurodegenerative Diseases
• Neuroregenerative Medicine
• Virology
• Infectious Diseases - Viral
• Immunological Signaling

The sensitivity of the Single Molecule Counting (SMC^®) technology gives the SMC^® Human IFN-β1 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IFN-β1 in human serum and plasma.

Leistungsmerkmale und Vorteile

This new streamline kit has the following features including:

• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO^® and Erenna

Verpackung

96 well plate

Komponenten

• Assay Buffer
• IFN-β1 Coated Beads
• Standard Diluent
• IFN-β1 Detection Antibody
• IFN-β1 Standard
• 10X Wash Buffer
• Buffer D
• Elution Buffer B

Rechtliche Hinweise

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Piktogramme: GHS06,GHS08
• Signalwort: Danger
• H-Sätze: H302 + H332,H311,H319,H360FD,H412
• P-Sätze: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313
• Gefahreneinstufungen: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
• Lagerklassenschlüssel: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects