800819C

18:0-22:6 DG

1-stearoyl-2-docosahexaenoyl-sn-glycerol, chloroform

• Synonym(e): 1-octadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycerol; DG(18:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)/0:0)
• Empirische Formel (Hill-System): C₄₃H₇₂O₅
• CAS-Nummer: 65886-80-8
• Molekulargewicht: 669.03
• MDL-Nummer: MFCD22570234
• UNSPSC-Code: 12352211
• NACRES: NA.25

Eigenschaften

• Assay: >99% (TLC)
• Form: liquid
• Verpackung: pkg of 1 × 5 mL (800819C-10mg)
• Hersteller/Markenname: Avanti Research^™ - A Croda Brand 800819C
• Konzentration: 2 mg/mL (800819C-10mg)
• Lipid-Typ: neutral lipids; neutral glycerides
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

In biochemical signaling, diacylglycerol (DAG) functions as a second messenger signaling lipid, and is a product of the hydrolysis of the phospholipid PIP2 (phosphatidylinositolbisphosphate) by the enzyme phospholipase C (PLC) (a membrane-bound enzyme) that, through the same reaction, produces inositol trisphosphate (IP3). Although inositol trisphosphate (IP3) diffuses into the cytosol, DAG remains within the plasma membrane due to its hydrophobic properties. IP3 stimulates the release of calcium ions from the smooth endoplasmic reticulum, whereas DAG is a physiological activator of protein kinase C (PKC). The production of DAG in the membrane facilitates translocation of PKC from the cytosol to the plasma membrane.
Diacylglycerol mimicks the effects of the tumor-promoting compounds phorbol esters.

Anwendung

18:0-22:6 DG or 1-stearoyl-2-docosahexaenoyl-sn-glycerol has been used:

• in the preparation of Golgi-like liposomes
• to study its effect on conventional protein kinase C (cPKC) and novel protein kinase C (nPKC) isozymes in vitro
• as a substrate for the measurement of diacylglycerol kinase η1 (DGKη1) activity in vitro

Verpackung

30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (800819C-10mg)

Lagerung und Haltbarkeit

Diacylglycerols are conveniently stored in chloroform solutions in glass vials with PTFE-lined caps at -20°C. Under these conditions acyl migration is minimal. Avoid plastic when handling chloroform solutions.

Sonstige Hinweise

Delivery to cells:
Dry samples of diacylglycerol in chloroform, using a stream of nitrogen. Dissolve the residue in an appropriate volume of ethanol or DMSO, then dilute to the desired aqueous medium.

Effective concentration:
Most biological responses saturate at 20 to 250 μM sn-1,2-dioctanoylglycerol. Only sn-1,2 isomers appear to be active.

Precaution: Since short chain Diacylglycerols mimic effects of the tumor-promoting phorbol diesters in a number of biological systems, extra care should be employed in their handling. Treatment of solutions, vessels and other articles with 1N NaOH before washing or discarding will destroy diacylglycerols.

Rechtliche Hinweise

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Sicherheitshinweise

• Piktogramme: GHS06,GHS08
• Signalwort: Danger
• H-Sätze: H302,H315,H319,H331,H336,H351,H361d,H372,H412
• P-Sätze: P201 - P273 - P301 + P312 + P330 - P302 + P352 - P304 + P340 + P311 - P308 + P313
• Gefahreneinstufungen: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3
• Zielorgane: Central nervous system, Liver,Kidney
• WGK: WGK 3