807745

APN-COCI

• Synonym(e): 4-(cyanoethynyl)benzoyl chloride
• Empirische Formel (Hill-System): C₁₀H₄ClNO
• Molekulargewicht: 189.60
• UNSPSC-Code: 12352101
• PubChem Substanz-ID: 329768747
• NACRES: NA.22

Eigenschaften

• Form: powder
• Qualitätsniveau: 100
• Eignung der Reaktion: reagent type: cross-linking reagent
• Lagertemp.: 2-8°C
• SMILES String: N#CC#CC1=CC=C(C(Cl)=O)C=C1
• InChI: 1S/C10H4ClNO/c11-10(13)9-5-3-8(4-6-9)2-1-7-12/h3-6H
• InChIKey: OWNJCJKKKZXTIX-UHFFFAOYSA-N

Beschreibung

Anwendung

APN-COCl is a bifunctional reagent appropriate for the introduction of APN function.

Sonstige Hinweise

The coupling between an amine and APN-COCl should be performed in dry organic solvents (e.g. THF) in the presence of a base (e.g. TEA). The resulting conjugate can be readily coupled with thiol-containing biomolecules using the following protocol:

• Dissolve the protein in the appropriate buffer with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
• Apply the appropriate amount of the stock solution of the APN-labeled molecule (1-5 molar eq. per free cysteine residue).
• Incubate at room temperature for 2 hours.

If necessary, purify the protein conjugate using size exclusion chromatography or ultrafiltration.

Sicherheitshinweise

• Piktogramme: GHS05,GHS07
• Signalwort: Danger
• H-Sätze: H302,H314
• P-Sätze: P260 - P270 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 4 Oral - Eye Dam. 1 - Skin Corr. 1B
• Lagerklassenschlüssel: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable