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HCT 116 Cell Line human

91091005, human colon, Epithelial-like

Sinónimos:

HCT116 Cells

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About This Item

UNSPSC Code:
41106514

Nombre del producto

HCT 116 Cell Line human, colon carcinoma, 91091005

biological source

human colon

packaging

tube of 5 μg 91091005-DNA-5UG
pkg of vial of cells 91091005-1VL

growth mode

Adherent

karyotype

Modal no. 45

morphology

Epithelial-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human colon carcinoma

Cell Line Description

One of 3 strains of malignant cells isolated from a male with colonic carcinoma. Cells are tumourigenic in nude mice and form colonies on agarose. Growth and plating efficiency are enhanced by using a feeder layer of murine fibroblasts.

Application

HCT 116 Cell Line has been used to:
  • study the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
  • study the effects of different amounts of transferrin within the polyethyleneimine (PEI)-small interfering RNA (siRNA) complexes on the uptake of fluorescently-labelled siRNA
  • determine the effect of PTS (polyamine transport system) in the selective uptake of Quilamine HQ1-44, an iron chelator
Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 10,12
D16S539: 11,13
D5S818: 10,11
D7S820: 11,12
THO1: 8,9
TPOX: 8
vWA: 17,22

Culture Medium

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Artículos

ECACC Nucleic Acids

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

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