LoVo Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87060101, human colon (supraclavicular lymph node metastasis), Epithelial

• Sinónimos: LoVo Cells
• UNSPSC Code: 41106514

Propiedades

• biological source: human colon (supraclavicular lymph node metastasis)
• packaging: tube of 5 μg 87060101-DNA-5UG; pkg of vial of cells 87060101-1VL
• growth mode: Adherent
• karyotype: Modal no. 49, (2n=46)
• morphology: Epithelial
• products: Carcinoembryonic antigen (CEA)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human colon adenocarcinoma

Cell Line Description

Derived from a metastatic tumour in the left supraclavicular region of a 56 year old male with adenocarcinoma of the colon. The cells produce carcino embryonic antigen (CEA).

Application

Human LoVo cell line is part of the three-dimensional (3D) heterotypic spheroid model developed to assess cancer immunotherapy agents in vitro. It has been used to test the in vitro cytotoxic property and anti-proliferative property of C. lechleri stem bark essential oil.

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13,14
D13S317: 8,11
D16S539: 9,12
D5S818: 11,13
D7S820: 10,11
THO1: 9.3
TPOX: 8,9
vWA: 17,18

Culture Medium

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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