A dual wavelength-activatable gold nanorod complex for synergistic cancer treatment.

A dual wavelength-activatable gold nanorod complex for synergistic cancer treatment.

Nanoscale (2015-07-01)

Dennis B Pacardo, Bhanu Neupane, S Michaela Rikard, Yue Lu, Ran Mo, Sumeet R Mishra, Joseph B Tracy, Gufeng Wang, Frances S Ligler, Zhen Gu

PMID26122945

ABSTRACT

A multifunctional gold nanorod (AuNR) complex is described with potential utility for theranostic anticancer treatment. The AuNR was functionalized with cyclodextrin for encapsulation of doxorubicin, with folic acid for targeting, and with a photo-responsive dextran-azo compound for intracellular controlled drug release. The interaction of a AuNR complex with HeLa cells was facilitated via a folic acid targeting ligand as displayed in the dark-field images of cells. Enhanced anticancer efficacy was demonstrated through the synergistic combination of promoted drug release upon ultraviolet (UV) light irradiation and photothermal therapy upon infrared (IR) irradiation. This multifunctional AuNR-based system represents a novel theranostic strategy for spatiotemporal delivery of anticancer therapeutics.

MATERIALS

Product Number

Brand

Product Description

74875

Sigma-Aldrich

8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt, suitable for fluorescence, ≥90% (HPCE)

D80800

Sigma-Aldrich

N,N′-Dicyclohexylurea, 98%

270563

Supelco

Dichloromethane, ≥99.9%

T8154

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Trypan Blue solution, 0.4%, liquid, sterile-filtered, suitable for cell culture

85193

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719234

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1-Adamantanethiol, 99% (GC)

H5882

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Hexadecyltrimethylammonium bromide, ≥98%

52365

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Hexadecyltrimethylammonium bromide, BioUltra, for molecular biology, ≥99.0% (AT)

H9151

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Hexadecyltrimethylammonium bromide, BioXtra, ≥99%

H6269

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Hexadecyltrimethylammonium bromide, for molecular biology, ≥99%

493511

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Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%

213462

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Sodium borohydride, ReagentPlus^®, 99%

452874

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Sodium borohydride, granular, 10-40 mesh, 98%

452882

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480886

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452890

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71320

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Sodium borohydride, purum p.a., ≥96% (gas-volumetric)

248762

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Silver nitrate on silica gel, extent of labeling: ~10 wt. % loading, +230 mesh

270997

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Dichloromethane, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer

D1435

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Dimethyl sulfoxide, meets EP testing specifications, meets USP testing specifications

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D8418

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D4540

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276855

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41639

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Sigma-Aldrich

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Sigma-Aldrich

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